Two papers will be presented:
1. "Nanog Is the Gateway to the Pluripotent Ground State"
Jose Silva, Jennifer Nichols, Thorold W. Theunissen, Ge Guo, Anouk L. van Oosten, Ornella Barrandon, Jason Wray, Shinya Yamanaka, Ian Chambers, and Austin Smith
Cell 138, 722-737, August 21, 2009
2. "Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells"
Elayne M Chan, Sutheera Ratanasirintrawoot, In-Hyun Park, Philip D Manos, Yuin-Han Loh, Hongguang Huo, Justine D Miller, Odelya Hartung, Junsung Rho, Tan A Ince, George Q Daley & Thorsten M Schlaeger
Nature Biotechnology Advance Online Publication
We will discuss the approaches to obtain authentic iPS cells and the markers that should be used to assure yourself you are working with trully pluripotent cells.
In addition to these papers, please come prepared to discuss the methods your group is using or considering to validate iPS cells.
About this ongoing activity:
The iPSC Interest Group Meeting is a monthly "journal club" like meeting for all researchers interested in using induced pluripotent stem cells in their studies. There is an emphasis on attendance for trainees. VCSCB faculty members, Aaron Bowman, Ph.D. and Kevin Ess, M.D., Ph.D., are co-sponsors. The meeting will focus on the nitty-gritty of applying iPSC technology to scientific questions. The objective is to build an institutional-wide collaborative group to build on each other's experiences and method development. iPSC meetings are held on the second Thursday each month from 3:00-4:00 PM in 6131 MRB III.