Downloading Current Guidelines
The Mouse and ESC Shared Resource provides updated guidelines that are of relevant use for those preparing samples for, or analysing samples from, the Shared Resource. You may download these guidelines in PDF format.Outline of Current Guidelines
I. Design of Gene Targeting Vectors
A. General considerations
B. Strain origin of mouse DNA
C. Recombineering to build targeting vectors
D. Specific considerations
II. Choice of Embryonic Stem Cell Lines
A. Discussion of available lines
B. Frequently asked questions about ESCs
III. BAC Recombineering and Transgenesis
A. Bacterial Artificial Chromosomes (BACs) and Recombineering: A primer
B. How to use BACs
C. Considerations for selecting BACs and handling BAC DNA
D. How to locate BACs on genome browsers
E. Web Links
F. Protocols and Reagents
IV. References for Sections I-III.
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