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Services and Pricing

Assisted Reproduction Technologies

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When new transgenic lines need to be introduced into animal facilities, or when animals need to be rederived to remove specific pathogens, this Shared Resource is capable of performing a variety of techniques necessary to prevent the introduction of pathogens in the mouse facilities. This includes in vitro fertilization, rederivation and ovarian transplantation.

In vitro fertilization requires fresh or frozen sperm from a transgenic male that is used to fertilize oocytes from superovulated female mice. After incubation overnight and fertilization, two-cell embryos are transferred into recipient animals. About 19 days later, pups are born and at 3 weeks of age the Transgenic Core staff will wean, tail, and ear punch the pups. The investigator will then screen the tail DNA for transgenic founders and notify the core of their results.

For rederivations, fresh or frozen 0.5 to 3.5 day old embryos are washed numerous times in sterile medium and are then transferred into recipient females. Seventeen to nineteen days later pups are born and at 3 weeks of age the Transgenic Core staff will wean, tail, and ear punch the pups. The investigator will then screen the tail DNA for transgenic founders and notify the core of their results.

For ovary transplantation, ovaries from a transgenic female are removed and transplanted into 1-2 recipient animals. This service, although not frequently utilized, is essential when a valuable line of animals ceases to reproduce and is at risk.

In Vitro fertilization Rederivation Ovary transplantation
  1. PI submits DNA and appropriate service form to TMESCSR
  2. Service forms are reviewed and approved by the Co-Directors of the resource
  3. IVF is scheduled, mice are ordered
  4. IVF is performed
  5. The following day, fertilized embryos are transferred to pseudopregnant females
  6. Pups born 19 to 20 days post ferilization
  7. Pups weaned, tailed and ear punched by Transgenic Core staff at 3 weeks of age
  1. PI submits appropriate service form to TMESCSR
  2. Service forms are reviewed and approved by the Co-Directors of the resource
  3. Rederivation is scheduled, mice are ordered (if required)
  4. Rederivation is performed, embryos are transferred to pseudopregnant females
  5. Pups born 17 to 20 days following transfer
  6. Pups weaned, tailed and ear punched by Transgenic Core staff at 3 weeks of age
  7. PI identifies transgenic founders from tail DNA using PCR and Southern blotting
  8. PI notifies Tg Core staff about transgenic founders
  9. Non-transgenic littermates are serology tested at 6 weeks of age
  10. DAC staff review serology results, approve movement of mice, and transfer transgenic founders to PIs mouse housing room at 7 weeks of age
  1. PI submits appropriate service form to TMESCSR
  2. Service forms are reviewed and approved by the Co-Directors of the resource
  3. Ovary transplatation is scheduled
  4. Ovary transplant is performed
  5. Mice will be housed in the barrier facility for 4 weeks
  6. Females will be serology tested after 4 weeks
  7. DAC staff review serology results, approve movement of mice, and perform transfer of mice to PI.s mouse housing room

Available Services

Assisted Reproduction Technologies
Cryopreservation of Mouse Embryos
ES Cell Microinjection
Gene Targeting
Karyotyping
Pronuclear DNA Microinjection
RMCE (Recombinase-mediated cassette exchange)
Sperm Cryopreservation