Services and Pricing

Cre or Flp^e Pronuclear DNA Microinjection

The resource performs these microinjections directly into fertilized embryos derived from gene-targeted mice, but males studs need to be provided by the investigator. Utilizing a supercoiled Cre or Flp^e expression plasmid, removal of "floxed" or "flrted" gene segments of DNA from the genome can be achieved. This charge for this service is $4,800. Currently, three core employees can perform Cre or Flp^e pronuclear DNA microinjections.

The investigator will be notified of potential transgenic pups as weaning of these pups nears. Pups will be tailed and numbered at 3 weeks of age by core personnel. The investigator will need to perform DNA isolation and analyze the DNA by PCR and Southern blotting. If no transgenic models are generated, a decision will need to be made as to the concentration of the Cre or Flp^e being injected.