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Karyotyping

Embryonic stem cells have a tendency to lose chromosomes over time in culture. Needless to say, these large genetic losses are usually incompatible with germline transmission of the ESC genome. Therefore, we have established methods for quickly determining the chromosome number present in targeted mESC lines. Information gained from this procedure allows us to choose which clones are best among different correctly targeted clones. The normal mouse karyotype consists of 40 chromosomes. Obtaining the karytoype of clones to be injected into blastocyst is highly useful information since it helps to maximize the chances of germline transmission by selecting clones with the highest percentage of normal cells.