Services and Pricing

Cryopreservation of Mouse Embryos

Cryopreservation provides a safe path for perserving the quality and protection of the transgenic line for any potential projected future experiments. The investigator is responsible for providing a complete description of the mouse line to be cryopreserved (strain, genotype, etc.) Wildtype (purchased) female mice are superovulated and mated with a minimum of 8 heterozygous or 7 homozygous male stud mice between the ages of 2-6 months that are provided by the investigator. From these matings a minimum of 300 heterozygous or 150 homozygous embryos are harvested and cryopreserved.

Alternatively, embryos can be cryopreserved after performing an in vitro fertilization. Fresh or frozen sperm is cultured with wildtype eggs donated from superovulated females. The resulting fertilized embryos can be used for embryo cryopreservation or transferred to produce live pups.

Frozen embryos are stored in liquid nitrogen storage tanks. A yearly storage fee applies for each line. These tanks are monitored weekly by personnel of the resource, and cannot be accessed by other individuals.